Molecular Neurobiology Laboratory

Molecular Neurobiology Lab Group

Lab Description

We are working on new strategies for organ protection by boosting highly conserved signaling pathways that Nature has designed to protect cells from various forms of stress-induced damage. Small ubiquitin-like modifier (SUMO1-3) conjugation is an archetypical signaling pathway that is known to protect cells from various kinds of stress. Our data support the notion that targeting SUMO conjugation is a promising new strategy for protecting organs in the clinical setting.Our long-term goal is to improve outcomes in patients who suffer a stroke or other forms of brain ischemic injury.

SUMO Research Projects

Major SUMO Research Projects

Wulf Paschen, PhD
Wulf Paschen, PhD

Laboratory Director
Professor in Anesthesiology

Wei Yang, PhD
Wei Yang, PhD
Assistant Professor

Shuai Liu, PhD
Shuai Liu, PhD
Postdoctoral Associate

Pei Miao
Pei Miao
Research Technician II

  1. Subramonian D, Raghunayakula S, Olsen JV, Beningo KA, Paschen W, Zhang X-D. Analysis of changes in SUMO2/3 modification during breast cancer progression and metastasis. J Proteome Res; 29 Jul 2014; DOI: 10.1021/pr500119a.
  2. Wang L, Wansleeben C, Zhao S, Miao P, Paschen W, Yang W. SUMO2 is essential while SUMO3 is dispensable for mouse embryonic development. EMBO Rep. 2014;15:878-885.
  3. Iwabuchi M, Sheng H, Thompson JW, Wang L, Debois LG, Gooden D, Moseley MA, Paschen W, Yang W. Characterization of the ubiquitin-modified proteome regulated by transient forebrain ischemia. J Cereb Blood Flow Metabol. 2014;34:425-432.
  4. Yang W, Sheng H, Thompson JW, Zhao S, Wang L, Miao P, Liu X, Moseley MA, Paschen W. Small ubiquitin-like modifier 3-modified proteome regulated by brain ischemia in novel small ubiquitin-like modifier transgenic mice: putative protective proteins/pathways. Stroke. 2014;45:1115-1122.
  5. Wang L, Rodriguiz RM, Wetsel WC, Sheng H, Zhao S, Liu X, Paschen W, Yang W. Neuron-specific SUMO1-3 knockdown in mice impairs episodic and fear memories. J Psychiatry Neurosci. 2014;39:259-266.
  6. Yang W, Wang L, Paschen W. Development of a high-throughput screening assay for inhibitors of small ubiquitin-like modifier proteases. J Biomol Screen. 2013;18:621-628.
  7. Yang W, Wang L, Roehn G, Pearlstein RD, Ali-Osman F, Pan H, Goldbrunner R, Krants M, Harms C, Paschen W. Small ubiquitin-like modifier 1-3 conjugation is activated in human astrocytic brain tumors and is required for glioblastoma cell survival. Cancer Sci. 2013;104:70-77.
  8. Wang L, Ma Q, Yang W, Mackensen GB, Paschen W. Moderate hypothermia induces marked increase in levels and nuclear accumulation of SUMO2/3-conjugated proteins in neurons. J Neurochem. 2012;123:349-359.
  9. Yang W, Thompson JW, Wang Z, Wang L, Sheng H, Foster MW, Moseley MA, Paschen W. Analysis of oxygen/glucose-deprivation-induced changes in SUMO3 conjugation using SILAC-based quantitative proteomics. J Proteome Res. 2012;11:1108-1117.
  10. Datwyler AL, Laettig-Tuennemann G, Yang W, Paschen W, Lee SL, Dirnagl U, Endres M, Harms C. SUMO2/3 conjugation is an endogenous neuroprotective mechanism. J Cereb Blood Flow Metabol. 2011;31:2152-2159.
  11. Yang W, Ma Q, Mackensen GB, Paschen W. Deep hypothermia markedly activates the small ubiquitin-like modifier conjugation pathway; implications for the fate of cells exposed to transient deep hypothermic cardiopulmonary bypass. J Cereb Blood Flow Metabol. 2009;29:886-890.
  12. Yang W, Sheng H, Homi HM, Warner DS, Paschen W. Cerebral ischemia/stroke and small ubiquitin-like modifier (SUMO) conjugation – a new target for therapeutic intervention? J Neurochem. 2008;106:989-999.
  13. Yang W, Sheng H, Warner DS, Paschen W. Transient focal cerebral ischemia induces a dramatic activation of small ubiquitin-like modifier conjugation. J Cereb Blood Flow Metabol. 2008;28:892-896.
  14. Yang W, Sheng H, Warner DS, Paschen W. Transient global cerebral ischemia induces a massive increase in protein sumoylation. J Cereb Blood Flow Metabol. 2008;28:269-279.

Wulf Paschen, PhD

Wulf Paschen, PhD

Professor in Anesthesiology
Research Professor in Neurobiology
Director, Molecular Neurobiology Laboratory

Contact Us

DUMC 3094, MS #28
130 Sands Building
303 Research Drive
Durham, NC 27710

Office: 919-684-5576
Fax: 919-684-6692