The Nackley Laboratory

The Nackley Laboratory
The Nackley Laboratory Team

Opportunities in the Lab

We are seeking a highly motivated scientist to join our group as a LAB RESEARCH ANALYST II. Details on how to apply can be found on the Duke HR website.

Third Year Medical Program: The Third Year at the Duke University School of Medicine represents a unique opportunity for medical students to broaden their background in the basic sciences. If you are a Duke University 3rd year medical student, and interested in a one-year research experience focusing on chronic pain, please contact Dr. Andrea Nackley (andrea.nackley@duke.edu; 919.613.5911) to join this dynamic lab.

Shepherd SD, O’Buckley SC, Harrington JM, Haines LG, Rothrock GD, Johnson LM, Nackley AG. A Moldable Sustained Release Bupivacaine Formulation for Tailored Treatment of Postoperative Dental Pain. Sci Rep. 2018 Aug 15;8(1):12172.

Ji RR, Nackley A, Huh Y, Terrando N, Maixner W. Neuroinflammation and Central Sensitization in Chronic and Widespread PainAnesthesiology. 2018 Aug;129(2):343-366.

Kim S, Zhang X, O’Buckley SC, Cooter M, Park JJ, Nackley AG. Acupuncture Resolves Persistent Pain and Neuroinflammation in a Mouse Model of Chronic Overlapping Pain Conditions. J Pain. 2018 Jul 4. pii: S1526-5900(18)30305-5. [Epub ahead of print]

Zhang X, Hartung JE, Bortsov A, Kim S, O’Buckley SC, Kozlowski J, Nackley AG. Sustained Stimulation of β2- and β3-adrenergic Receptors Leads to Persistent Functional Pain and Neuroinflammation. Brain, Behavior, and Immunity. 2018 June 20 [Epub ahead of print].

Ji RR, Nackley A, Huh Y, Terrando N, Maixner W. Neuroinflammation and Central Sensitization in Chronic and Widespread Pain. Anesthesiology. 2018 Feb 19. [Epub ahead of print]

Harmon JB, Sanders AE, Wilder RS, Essick GK, Slade GD, Hartung JE, Nackley AG. Circulating Omentin-1 and Chronic Painful Temporomandibular Disorders. J Oral Facial Pain Headache. 2016 Summer;30(3):203-209.

Ciszek BP, O’Buckley SC, Nackley AG. Persistent Catechol-O-methyltransferase-dependent Pain Is Initiated by Peripheral β-Adrenergic Receptors. Anesthesiology. 2016 May;124(5):1122-35.

Oladosua FA, Ciszek BP, O’Buckley SC, Nackley AG. Novel Intrathecal and Subcutaneous Catheter Delivery Systems in the Mouse. J Neurosci Methods. 2016 May 1;264:119-28. Epub 2016 Mar 11.

Nackley A. Chronic Pain: Translating Discoveries Into Treatments. International Innovation. 2015 June;185:56-58.

Congratulations to Dr. Andrea Nackley, one of the 2019 DIG award recipients for her proposal to explore the molecular relationship between chronic pain and obesity. In collaboration with the Division of Metabolic and Weight Loss Surgery, the study aims to help identify molecular features of adipose tissue expansion that underlie comorbid chronic pain and obesity.

The Duke Clinical & Translational Science Institute (CTSI) has chosen to fund through the Duke/RTI Collaborative Translational Research Grants program, the application submitted by Dr. Andrea Nackley. The study title is “A New Peripherally-restricted Adrb3 Antagonist for the Treatment of Chronic Pain and Inflammation” 

R01 Grant Awarded to Dr. Andrea Nackley
Title:
 Vestibulodynia: Understanding Pathophysiology and Determining Appropriate Treatments (Vestibulodynia: UPDATe)
Award Date: 9/11/2018
Congratulations to Andrea Nackley, PhD, on being awarded a 5-year, $3,846,094 NIH grant to conduct a multi-site clinical trial aimed at determining optimal therapies for patients suffering from a complex chronic vulvar pain condition.

26th Annual Duke Anesthesiology Academic Evening: May 29, 2018
1. Congratulations to Dr. Xin Zhang, whose abstract titled, “Activation of Peripheral β2 and β3ARs Leads to Increased Nociceptor Activity” wowed the judges and received first place in the Basic Sciences category.

2. Congratulations to Scott Scarneo, first place recipient of the best pre-doc, non-medical student category for his abstract titled, “Genetic and Pharmacological Validation of TAK1 Inhibition in Macrophages as a Therapeutic Strategy to Effectively Inhibit Inflammatory Pain.”

Julia Kozlowski and Dr Andrea Nackley at the April 2018 undergraduate thesis poster session Julia Kozlowski and Dr Xin Zhang at the April 2018 undergraduate thesis poster session

Congratulations to Julia Kozlowski, Duke senior undergraduate, on successfully presenting her scientific poster on April 24, 2018 as the concluding part for her Neuroscience Graduation with Distinction degree. Julia was mentored by Drs. Andrea Nackley and Xin Zhang, who also formed part of her thesis committee. Julia leaves our lab to become a medical student at the University of Michigan.

Dr. Xin Zhang receiving the Best Abstract Award for his poster titled “Activation of Peripheral β2 and β3ARs Leads to Increased Nociceptor Activity”at the 2018 Pain & Genetics SIG of the 37th annual APS meeting.

Dr. Xin Zhang at the APS Meeting in Anaheim.

Dr. Xin Zhang receiving the Best Abstract Award for his poster titled “Activation of Peripheral β2 and β3ARs Leads to Increased Nociceptor Activity”at the 2018 Pain & Genetics SIG of the 37th annual APS meeting.

The Duke School of Medicine Multicultural Resource Center has nominated first-year medical student Zachary Smothers as a recipient of the “Charles D Watts Travel Award.” Zachary will attend the 37th Annual Scientific Meeting of the American Pain Society, to be held March 4 -6, 2018, in Anaheim, CA, where he will attend pertinent lectures and hold discussions with experts in the chronic pain field. This experience is aimed to increase his ability to better serve patients from diverse backgrounds.

Dr. Xin Zhang has been nominated by the 2018 Pain & Genetics Shared Interest Group (SIG) of APS for the ‘Best Abstract Award’. He will receive the award March 5, 2018, following a short presentation of his poster, “Activation of Peripheral β2 and β3ARs Leads to Increased Nociceptor Activity.”

RO3 Grant Awarded to Dr. Andrea Nackley
Title: 
Defining the Role of Adipocyte Adrb3 in Chronic Pain
Award Date: January 9, 2018
Congratulations to Andrea Nackley, PhD, on receiving $160,000 in funding to study peripheral mechanisms that drive chronic pain and work to develop peripherally-restricted therapies for patients with functional pain syndromes from The National Institute of Neurological Disorders and Stroke (NINDS). READ MORE

2018 APS Young Investigator Travel Support Program awarded to Dr. Xin Zhang
Dr. Zhang will travel to, and present a poster at, the 37th Annual Scientific Meeting of the American Pain Society, held March 4 -6, 2018 in Anaheim, CA. APS gratefully acknowledges the National Center for Complementary and Integrative Health (NCCIH) for support of the Young Investigator Travel Award program. View Abstract

Dr. Andrea Nackley Appointed Co-Chair of Early Career Forum
The American Pain Society invited Dr. Nackley to become co-chair of the Early Career Forum. The appointment starts with co-planning the 2018 Scientific Summit with the current chair, continuing through 2019 when Dr. Nackley will be appointed the chair of the Early Career Forum.

Outstanding Poster Award awarded to Dr. Xin Zhang
Dr. Zhang received the award for his poster entitled, “Activation of Peripheral β2 and β3ARs Leads to Increased Nociceptor Activity” at the Translational Pain Research Symposium, Duke Kunshan University Kunshan, China, June 21-23, 2017.
Summary: Dr. Zhang showed that i) COMT inhibition leads to pain sensitivity, in line with increased ERK phosphorylation in DRG neurons and strengthened nociceptor activity in response to noxious stimuli, ii) COMT-dependent increases in pain sensitivity and nociceptor activity are driven by peripheral β2- and β3ARs, and iii) treatments targeted towards peripheral β2- and β3ARs and downstream effectors may prove useful in the management of functional pain syndromes.

2017 APS Young Investigator Travel Support Program awarded to Dr. Xin Zhang
Dr. Zhang will travel to, and present a poster at, the 36th Annual Scientific Meeting of the American Pain Society, held May 17-20, 2017 in Pittsburgh, Pennsylvania. APS gratefully acknowledges the National Center for Complementary and Integrative Health (NCCIH) for support of the Young Investigator Travel Award program.

Duke 2017 Summer Neuroscience Program Fellowship awarded to Katie Kanter
Title: Effects of MOR-1K Genetic Variation on Cellular Activity
Summary: Opioid induced hyperalgesia manifests as increased pain sensitivity due to acute or chronic opioid administration. A truncated variant in the mu opioid receptor, MOR-1K, has been linked to pain in human genetic studies, and shown to produce cellular excitation, resulting in hyperalgesia rather than analgesia.The Nackley Lab has identified a candidate single nucleotide polymorphism (SNP) within the enhancer box regulatory motif on MOR-1K exon 13 in CXB7/ByJ mice, that is predicted to contribute to the increased pain sensitivity observed in this variant compared to 129S6 mice. The proposed work will continue a previously unpublished study by the Nackley lab, to elucidate alterations to MOR-1K receptor function related to this SNP using a cAMP assay, and ultimately examine changes to transcriptional regulation via a luciferase assay.

NIH/NIDCR Grant # R56DE025296-01 awarded to Dr. Andrea Nackley
Title: Proteins, MicroRNAs and Genes Associated with TMD and Overlapping Conditions
Awarded Date: September 21, 2016
Summary: The Institute of Medicine found that chronic pain affects 100 million Americans, causing extensive economic, social, and personal costs. For some the pain remains localized, while for others the pain spreads to affect multiple anatomic sites, suggesting a common underlying cause. In response to PA-14-244, we plan to use stored biospecimens and existing data from a clinical study of 1,460 adults to determine biological (proteins, microRNAs, and gene polymorphisms), psychosocial (stress, depression, anxiety), and clinical (general health and environmental exposures) factors that contribute to localized and overlapping pain conditions. Further, we will use bioinformatics methods to understand how these factors interact to influence pain, with the long-term goal to identify biomarkers for the diagnosis and treatment of chronic overlapping pain conditions.

Brittney Ciszek, PhD Receiving her Academic Evening Award
Brittney Ciszek PhD, is the first-place recipient of the 24th Annual Duke Anesthesiology Academic Evening’s Excellence in the Pre-Doctoral Non-Medical Student category.

Bomi Oladosu has been selected to receive the GPSF Excellence in Mentoring Award 2016. The award will be presented at the 18th Annual Graduate School Student Recognition Ceremony, scheduled for April 14 at the George Watts Hill Alumni Center, University of North Carolina at Chapel Hill.

F31 from NIAMS/NIH awarded to Jane Hartung
Title: The Role of TNF-alpha and MAP Kinases in the Maintenance of COMT-Dependent Pain
Date: 09/01/2015 – 08/31/2017
Summary: This proposal utilizes a unique animal model that mimics the genetics and physiology of chronic musculoskeletal pain conditions along with behavioral pharmacologic, immunocytochemical, and live animal imaging techniques to test the hypothesis that tumor necrosis factor alpha (TNFα) and mitogen-activated protein kinases (MAPKs) maintain COMT-dependent pain at cellular and behavioral levels.

Sandra O’Buckley
Lab Research Analyst I
Nackley Laboratory Manager
Email: sandra.obuckley@duke.edu
Office: +1-919-684-4867
Fax: +1-919-613-2324

Center for Translational Pain Medicine
Department of Anesthesiology
Duke University

# 6290 MSRB III
3 Genome Court
DUMC 3094, MS #50
Durham, NC 27710

Chris KeithThe Nackley Laboratory